This is a fast mass spectroscopic technique with a large mass range, ideal for the analysis of anything from low mass molecules, fragile macromolecules, and peptide, lipid or glycan conjugates to synthetic polymers and proteins. The soft-ionization method means that samples can be ionized without fragmentation, allowing for detection of intact mass.  Typically, analytes are mixed with matrix in solution. A small amount of this is deposited on a stainless steel target and allowed to dry. The resulting solid residue is analyzed by the MALDI directly. It is also possible to analyze samples without matrix and to analyze some insoluble compounds. The instrument can be upgraded to analyze TLC plates and do imaging.

Mass Spectrometer Specs:

• Mass Range: up to 500,000 m/z
• Two modes of operation: Linear Mode for large molecules > 5 kDa and Reflectron Mode (better resolution) for small molecules and peptides. 
• Uses a SmartBeam-II laser technology, 355 nm wavelength and up to 2 kHz repetition rate. 
• Has both positive and negative ion modes, with ion acceleration up to +20/-20 kV. 
• Uses a High dynamic range FlashDetector system with a 10 bit 5 Gs/s digitizer.
• Mass Accuracy

Linear Mode With external calibrant   ≤ 100 ppm

With internal calibrant  ≤ 90 ppm

Reflectron Mode With external calibrant  ≤ 10 ppm

With internal calibrant  ≤ 2 ppm

Rates:

Instrument use:  $48/h for CWRU and academic institutions, $100/h for industry.

Staff time (includes sample workup, spotting on the target, running on instrument, and training for non-CWRU users):  $52/h for academic institutions and $100/h for industry.

Note: All rates are charged in 15 min increments. 

To use the instrument, you must first be trained and cleared by our staff. Training is free for all CWRU users. To get trained or if you would like our staff to run your samples, please contact our engineer:

James Faulk

jdf4@case.edu
216.368.3589
Millis Hall 224A

To schedule time on the instrument, please click HERE (file available only to trained/cleared users)

The purchase of this instrument was made possible by the National Science Foundation under Grant No. 2320090. For Publications and presentations supported by this award, please acknowledge NSF support using the suggested working: “This material is based upon work supported by the National Science Foundation under Grant No. 2320090.”